for visualizing alpha diversity.
shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.
shows that taxonomy prediction accuracy is <50% for V4 sequences.
shows low accuracy of closed- and open-ref. QIIME OTUs.
shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.
UPARSE tutorial video
posted on YouTube. Make OTUs from MiSeq reads.
New in v11
OTU errors and biases
Errors and biases in amplicon sequencing
Spurious OTUs in mock and real samples
Tolstoy's paradox, spurious clusters are common with low error rates
Abundance bias (read count does not correlate with species abundance)