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Video talks
on 16S data analysis posted.
URMAP
ultra-fast read mapper posted (
paper)
.
~20% of taxonomy annotations in SILVA and Greengenes are wrong (
paper
).
Taxonomy prediction is <50% accurate for 16S V4 sequences (
paper
).
97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (
paper
).
USEARCH
Download 32-bit
Buy 64-bit
New in v11
Tech support
Documentation
Commands
Topics
OTU analysis
Sequence search
Sequence clustering
Taxonomy
Diversity
Octave plots
Machine learning
Chimeras
Read quality
Paired reads
OTU errors and biases
Publications
Clustering
UPARSE (OTU clustering for next-gen amplicon reads)
UCLUST, general-purpose sequence clustering
UCLUST sort order
Abundance sorting
Agglomerative clustering (single, complete and average linkage)
Connected components (single-linkage clustering)
Can UCLUST cluster metagenomics contigs?
Clustering sequences using local alignment