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11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.




See also
UNOISE algorithm
  unoise3 command

Denoising, also called noise reduction, is a generic term for a process that removes noise from a signal (see Wikipedia article). In next-generation sequencing, denoising generally refers to a computational method for removing sequence errors from amplicon reads or, equivalently, identifying the correct biological sequences in the reads.