UCLUST sort order
Sort sequences by decreasing size
annotation, which usually refers to the size of a cluster. The
size is specified by a field size=N; in the sequence label, where N is an integer.
The ‑minsize and -maxsize
options can be used to specify a minimum and maximum size, respectively.
Output is written to a FASTA file using the -fastaout option and/or a FASTQ file
using the -fastqout option.
For most applications, it is recommended that sequences
are globally trimmed before clustering and
The -relabel prefix option can be used to
generate sequential labels for the sorted sequences. The output label is prefixN where N=1, 2, 3 etc. If -sizeout is
used, a size annotation will be appended to the
The -topn N option specifies that no
more than N sequences should be output.
usearch -sortbysize seqs.fasta -fastaout seqs_sorted.fasta -minsize 4