OTU QC: Low-complexity sequence
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11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

24-Nov-2016
UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.

 

USEARCH v11

OTU QC: Low-complexity sequence

See also
  Quality control for OTU sequences

Low-complexity reads may be generated during sequencing. For example, Illumina reads typically contain low-complexity noise if they continue past the reverse sequencing adapter due to a short construct. Low-complexity sequence can be detected and filtered using the filter_lowc command.