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USEARCH v11

Estimating read depth (sampling effort) from an octave plot

See also
  Octave plot
  Interpreting an octave plot
  Log-normal abundance distribution
  otutab_octave command

Doubling the number of reads moves the distribution one bin to the right in an octave plot. You can estimate the number of additional reads required to sample the full richness by assuming that the distribution is approximately symmetrical, as in the example below. Here, we would need three to four additional bins to capture all OTUs, which corresponds to 23 to 24 = 8 to 16 times more reads.