Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

Commands for diversity analysis

  alpha_div  Calculate alpha diversity metric(s) from OTU table
  alpha_div_rare  Calculate alpha diversity metric(s) from OTU table with rarefaction
  alpha_div_sig  Statistical significance of alpha diversity correlation with metadata
  beta_div  Calculate beta diversity metric(s) from OTU table