for visualizing alpha diversity.
shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.
shows that taxonomy prediction accuracy is <50% for V4 sequences.
shows low accuracy of closed- and open-ref. QIIME OTUs.
shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.
UPARSE tutorial video
posted on YouTube. Make OTUs from MiSeq reads.
New in v11
OTU errors and biases
Extract sequences from a
in FASTA format. Sequences are masked according to the settings used to created the UDB file.
usearch -udb2fasta db.udb -output db.fasta