fasta_explode command
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11-Aug-2018 New paper describes octave plots for visualizing alpha diversity.

12-Jun-2018 New paper shows that one in five taxonomy annotations in SILVA and Greengenes are wrong.

18-Apr-2018 New paper shows that taxonomy prediction accuracy is <50% for V4 sequences.

05-Oct-2017 PeerJ paper shows low accuracy of closed- and open-ref. QIIME OTUs.

22-Sep-2017 New paper shows 97% threshold is wrong, OTUs should be 99% full-length 16S, 100% for V4.

UPARSE tutorial video posted on YouTube. Make OTUs from MiSeq reads.



fasta_explode command

The fasta_explode command creates one FASTA file for each sequence in the input file, which must be in FASTA format. Output file names are the sequence labels in the input file, so every label must be a valid file name for your operating system, otherwise the command will fail.


usearch -fasta_explode db.fa