Video talks on 16S data analysis posted.

URMAP ultra-fast read mapper posted (paper).

~20% of taxonomy annotations in SILVA and Greengenes are wrong (paper).

Taxonomy prediction is <50% accurate for 16S V4 sequences (paper).

97% OTU threshold is wrong for species, should be 99% for full-length 16S, 100% V4 (paper).

New in v11

fastx_syncpairs command

The fastx_syncpairs command sorts paired reads so that the reads appear in the same order in both files. If a read label appears in one file and not the other, it is discarded. The input files can be in FASTA or FASTQ format.

Example

usearch -fastx_syncpairs fwd.fq -reverse rev.fq -output fwd_sorted.fq -output2 rev_sorted.fq