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-orfstyle


The orfstyle and mincodons options control how ORFs are identified by USEARCH. These options are used by the findorfs command and by translated searches .

The value of orfstyle is an integer obtained by adding the numbers from the table below. The default value of orfstyle is 5.

Value Description
1 Allow an ORF to start at the beginning of the nucleotide sequence (otherwise, must begin with a START codon).
2 Allow an ORF to start immediately following a STOP codon (otherwise, must begin with a START codon). This compensates for read errors that might corrupt a START codon sequence, and allows non-standard START codons.
4 Allow an ORF to end at the end of the nucleotide sequence (otherwise, must be terminated by a STOP codon).
8 Include the STOP codon, if any, in the translated sequence. This is useful in the findorfs command to distinguish ORFs that contain a STOP from those that are terminated by the end of the nucleotide sequence. The STOP codon is represented by an asterisk (*). Do not use for translated searches.
1sco
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