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MUSCLE manual
Clustering (tree-building) options

See also
Tree output files

The -cluster1 option applies to the first iteration , -cluster2 applies to the second iteration, -cluster applies to iterations 1 and 2. Values for these options are shown in the table below.
Value Description
upgma Fast O(N 2 ) approximation to the textbook UPGMA algorithm.
upgmb Variant of UPGMA (see papers). This is used by default for both iteration 1 and 2.
neighborjoining The Neighbor-Joining algorithm. Better at making phylogenetic trees, but typically worse for making guide trees for progressive alignment.Also slower. Usually not recommended.
1sco
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