4701 Reads 4663 Hits (99.2%) 252 Avg read length (min 249, max 254) 35 Reads with > 3% errors (0.745%) 0.151% Mean subst. error rate (1772 errs, 1174911 bases) 0.000% Mean insert error rate (0 errs, 1174911 bases) 0.002% Mean delete error rate (20 errs, 1174911 bases) Q Bases Pct Diffs Pex Pobs Qobs -- ---------- ------- ---------- ------- ------- ----- 3 456 0.04% 175 0.50119 0.38377 4.16 4 635 0.05% 245 0.39811 0.38583 4.14 5 709 0.06% 260 0.31623 0.36671 4.36 6 190 0.02% 46 0.25119 0.24211 6.16 7 153 0.01% 51 0.19953 0.33333 4.77 8 86 0.01% 16 0.15849 0.18605 7.30 9 135 0.01% 24 0.12589 0.17778 7.50 10 220 0.02% 31 0.10000 0.14091 8.51 11 140 0.01% 9 0.07943 0.06429 11.92 12 193 0.02% 6 0.06310 0.03109 15.07 13 211 0.02% 9 0.05012 0.04265 13.70 14 233 0.02% 6 0.03981 0.02575 15.89 15 172 0.01% 7 0.03162 0.04070 13.90 16 407 0.03% 24 0.02512 0.05897 12.29 17 264 0.02% 3 0.01995 0.01136 19.44 18 521 0.04% 11 0.01585 0.02111 16.75 19 571 0.05% 8 0.01259 0.01401 18.54 20 814 0.07% 10 0.01000 0.01229 19.11 21 926 0.08% 9 0.00794 0.00972 20.12 22 860 0.07% 6 0.00631 0.00698 21.56 23 1172 0.10% 6 0.00501 0.00512 22.91 24 1744 0.15% 2 0.00398 0.00115 29.41 25 1841 0.16% 4 0.00316 0.00217 26.63 26 1896 0.16% 1 0.00251 0.00053 32.78 27 636 0.05% 0 0.00200 0.00000 41.00 28 28 0.00% 0 0.00158 0.00000 41.00 29 405 0.03% 0 0.00126 0.00000 41.00 30 611 0.05% 2 0.00100 0.00327 24.85 31 735 0.06% 11 0.00079 0.01497 18.25 32 5115 0.44% 26 0.00063 0.00508 22.94 33 7456 0.63% 30 0.00050 0.00402 23.95 34 6603 0.56% 31 0.00040 0.00469 23.28 35 699 0.06% 5 0.00032 0.00715 21.46 36 1471 0.13% 11 0.00025 0.00748 21.26 37 1005 0.09% 10 0.00020 0.00995 20.02 38 854 0.07% 5 0.00016 0.00585 22.32 39 510 0.04% 11 0.00013 0.02157 16.66 40 135 0.01% 3 0.00010 0.02222 16.53 41 1134099 96.53% 658 0.00008 0.00058 32.36 Q to QObs tabbed: 3 4.2 4 4.1 5 4.4 6 6.2 7 4.8 8 7.3 9 7.5 10 8.5 11 11.9 12 15.1 13 13.7 14 15.9 15 13.9 16 12.3 17 19.4 18 16.8 19 18.5 20 19.1 21 20.1 22 21.6 23 22.9 24 29.4 25 26.6 26 32.8 27 41.0 28 41.0 29 41.0 30 24.9 31 18.2 32 22.9 33 24.0 34 23.3 35 21.5 36 21.3 37 20.0 38 22.3 39 16.7 40 16.5 41 32.4 EE N Diffs Div AvgE -- ---------- ---------- ------ ----- 0 4406 907 0.00% 0.21 1 126 197 0.40% 1.56 2 48 131 0.79% 2.73 3 30 116 1.19% 3.87 4 23 147 1.59% 6.39 5 9 52 1.98% 5.78 6 8 85 2.38% 10.62 7 6 59 2.78% 9.83 8 4 52 3.17% 13.00 11 2 32 4.37% 16.00 12 1 14 4.76% 14.00 Predicted by EE: MeanDiffs 0.38 MeanDiv 0.15% Reads >3% diverged 7 (0.0015%) Poisson lambda = 0.18 Diffs Div N_obs Pct_obs N_poiss Pct_poiss ----- ------ ---------- -------- ---------- --------- 0 0.00% 3796 81.407% 3898.8 83.611% 1 0.40% 568 12.181% 697.9 14.966% 2 0.79% 123 2.638% 124.9 2.679% 3 1.19% 70 1.501% 11.2 0.240% 4 1.59% 34 0.729% 0.7 0.014% 5 1.98% 16 0.343% 0.0 0.001% 6 2.38% 15 0.322% 0.0 0.000% 7 2.78% 6 0.129% 0.0 0.000% 8 3.17% 7 0.150% 0.0 0.000% 9 3.57% 4 0.086% 0.0 0.000% 10 3.97% 4 0.086% 0.0 0.000% 11 4.37% 3 0.064% 0.0 0.000% 12 4.76% 2 0.043% 0.0 0.000% 13 5.16% 1 0.021% 0.0 0.000% 14 5.56% 3 0.064% 0.0 0.000% 15 5.95% 6 0.129% 0.0 0.000% 16 6.35% 1 0.021% 0.0 0.000% 17 6.75% 3 0.064% 0.0 0.000% 19 7.54% 1 0.021% 0.0 0.000% 35 reads with 8 - 20 diffs Diffs tabbed: Diffs Div N Pct 0 0.00 3796 81.407 1 0.40 568 12.181 2 0.79 123 2.638 3 1.19 70 1.501 4 1.59 34 0.729 5 1.98 16 0.343 6 2.38 15 0.322 7 2.78 6 0.129 8 3.17 7 0.150 9 3.57 4 0.086 10 3.97 4 0.086 11 4.37 3 0.064 12 4.76 2 0.043 13 5.16 1 0.021 14 5.56 3 0.064 15 5.95 6 0.129 16 6.35 1 0.021 17 6.75 3 0.064 18 7.14 0 0.000 19 7.54 1 0.021 20 7.94 0 0.000 21 8.33 0 0.000 22 8.73 0 0.000 23 9.13 0 0.000 24 9.52 0 0.000 25 9.92 0 0.000 26 10.32 0 0.000 27 10.71 0 0.000 28 11.11 0 0.000 29 11.51 0 0.000 30 11.90 0 0.000 31 12.30 0 0.000 Sub counts A C G T ---------- ---------- ---------- ---------- A | 306112 85 200 104 C | 70 209679 34 152 G | 292 77 406859 273 T | 98 162 225 250489 Base call probs A C G T ---------- ---------- ---------- ---------- A | 0.9987308 0.0002773 0.0006525 0.0003393 C | 0.0003334 0.9987806 0.0001620 0.0007240 G | 0.0007166 0.0001890 0.9984245 0.0006699 T | 0.0003905 0.0006455 0.0008965 0.9980675