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fastx_split command
The fastx_split command divides a FASTA or FASTQ file into roughly equal-sized pieces. The size of a piece is measured by the file size in bytes rather than the number of sequences or number of sequence letters.

The -splits n option specifies the desired number of pieces. You may get n+1 pieces.

The -outname option specifies a pattern for the output file names, which are always FASTA. Ther must be a '@' character in the pattern, which is replaced by the split number 1, 2, 3...


usearch -fastx_split db.fastq -splits 10 -outname split@.fa