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fastq_join command

See also
FASTQ files
  Quality scores
  FASTQ format options

The fastq_join command combines paired reads into single sequences by reverse-complementing the reverse read and inserting padding (default NNNNNNNN). If R1 is the forward read and R2 is the reversed read, the combined read is R1 NNNNNNNN RevComp(R2).

The forward (R1) read filename is given by the fastq_join option.

The reverse (R2) read filename is given by the -reverse option.

The output is written to a FASTQ file by specifying the -fastqout option and/or to a FASTA file given by -fastaout.


usearch -fastq_join fwd.fastq -reverse rev.fastq -fastaout join.fa