USEARCH manual > options > blast6out file

blast6out file

The blast6out filename option specifies an output file format that is compatible with the NCBI BLAST m8 and NCBI BLAST+ outfmt 6 formats. It is a tab-separated text file with one line per alignment. By convention I use the .b6 extension for files in this format. There are twelve fields, as shown in the table.   Field   Description 1   Query label. 2   Target (database sequence or cluster centroid) label. 3   Percent identity. 4   Alignment length. 5   Number of mismatches. 6   Number of gap opens. 7   1-based position of start in query. For translated searches (nucleotide queries, protein targets), query start<end for +ve frame and start>end for -ve frame. 8   1-based position of end in query. 9   1-based position of start in target. For untranslated nucleotide searches, target start<end for plus strand, start>end for minus strand. 10   1-based position of end in target. 11   E-value calculated using Karlin-Altschul statistics. 12   Bit score calculated using Karlin-Altschul statistics.