Unbias reference databases
unbias_dbs_v10.0.tar.gz (4.3 Mb)
Copy number reference (copynr.fa)
16S sequences. A typical label is:
The annotation format should be self-explanatory.
This database was created by downloading all finished microbial genomes from
Genbank and predicting 16S genes using the
search_16s command. For each genome, the number of 16S genes was
V4 primer mismatch database (v4diffs.fa)
sequences. A typical label is:
The v4diffs annotation gives the total number of
mismatches with V4F (GTGCCAGCMGCCGCGGTAA) and V4R (GGACTACHVGGGTWTCTAAT).
Mismatches were measured using the search_pcr
command. If you need reference data for a different primer pair, let me
The unbias reference databases were created by
Robert C. Edgar and are licensed under a
Creative Commons Attribution-ShareAlike 3.0 Unported License.